Pseudorabies virus manipulates mitochondrial tryptophanyl-tRNA synthetase 2 for viral replication.

The pseudorabies virus (PRV) is identified as a double-helical DNA virus responsible for causing Aujeszky's disease, which results in considerable economic impacts globally. The enzyme tryptophanyl-tRNA synthetase 2 (WARS2), a mitochondrial protein involved in protein synthesis, is recognized for its broad expression and vital role in the translation process. The findings of our study showed an increase in both mRNA and protein levels of WARS2 following PRV infection in both cell cultures and animal models. Suppressing WARS2 expression via RNA interference in PK-15 cells led to a reduction in PRV infection rates, whereas enhancing WARS2 expression resulted in increased infection rates. Furthermore, the activation of WARS2 in response to PRV was found to be reliant on the cGAS/STING/TBK1/IRF3 signaling pathway and the interferon-alpha receptor-1, highlighting its regulation via the type I interferon signaling pathway. Further analysis revealed that reducing WARS2 levels hindered PRV's ability to promote protein and lipid synthesis. Our research provides novel evidence that WARS2 facilitates PRV infection through its management of protein and lipid levels, presenting new avenues for developing preventative and therapeutic measures against PRV infections.

The portrayal of gender in Marvel and Star Wars media targeted towards children.

An abundance of previous research has investigated how gender has been portrayed within feature length films produced by Walt Disney Animation Studios, particularly those within the Disney princess franchise. However, the Disney corporation acquired the Marvel and Star Wars franchises in 2009 and 2012, respectively, which was likely a strategy for the corporation to obtain characters that would capture the imagination of boys and men. The current qualitative study explored how gender is portrayed by leading protagonists in these texts, utilising thematic analysis, which was necessary considering little is currently known in this domain. The researchers analysed series one of Avengers Assemble and series one of Star Wars Rebels. Interpretation of the data led to the development of several themes and subthemes based on the gendered portrayals within each series. Overall, the findings suggest that there was more overt gender stereotyping in Avengers Assemble when compared with Star Wars Rebels, meaning that the former could be particularly problematic for children who may replicate its messages. The current study has facilitated a greater understanding of the gendered messages that may be consumed by children who engage with Marvel and Star Wars media. Future research is needed to assess the relationship between such messages and children's behaviour.

Providing Hemodialysis in Unstable Areas: An Assessment and Framework for Effective Care.

Introduction: Providing hemodialysis to patients with kidney failure (KF) in conflict-affected areas poses a significant challenge. Achieving and sustaining reasonable quality hemodialysis operations in such regions necessitates a comprehensive approach. Methods: In the conflict area of Northwest (NW) Syria, a 3-phase project was initiated to address the quality of hemodialysis operations. The assessment phase involved the examination of infection prevention and control (IPC) protocols, staff training, medical protocols, individualized hemodialysis prescriptions, and laboratory testing capabilities. The second phase involved activities toward capacity building and implementing an action plan based on feasibility and sustainability. Results: The assessment phase revealed that only 7 of 14 centers had IPC protocols, and 8 centers provided IPC training for their staff. Furthermore, only 7 centers had medical protocols, and 5 used individualized hemodialysis prescriptions. Difficulties in testing for potassium was reported in 7 centers and the inability to perform hepatitis B and C serologies was reported in 3 centers. Only 2 centers adhered to machine and water treatment system maintenance guidelines, and 4 conducted daily water quality checks. Recommendations were formulated, and an action plan was developed for implementation in the second phase. The plan encompassed enhancements in IPC practices, medical protocols, record-keeping, laboratory testing, and equipment maintenance. Conclusion: This project underscores that hemodialysis services in conflict-affected areas do not meet the standards for quality care. It emphasizes the necessity of implementing a comprehensive framework that engages relevant stakeholders in defining and upholding quality care, a model that should be extended to other protracted conflict-affected regions.

Clinical Significance of Tryptophanyl-tRNA Synthetase 1 Gene Expression in Patients With Locally Advanced Gastric Cancer.

BACKGROUND/AIM: The tryptophanyl-tRNA synthetase 1 gene (WARS1), encodes a tryptophan-tRNA synthetase involved in the amino acidification of tryptophan-tRNA and has been reported to be involved in cancer cell growth, metastasis promotion, and drug resistance in a variety of cancers. This study investigated the clinical significance of WARS1 expression as a biomarker in gastric cancer tissues obtained from patients with locally advanced gastric cancer (GC) who underwent radical resection. PATIENTS AND METHODS: WARS1 expression in GC tissues and adjacent normal gastric mucosa of 253 patients with pStage II/III GC who underwent curative resection was determined using quantitative polymerase chain reaction (PCR). Association of WARS1 expression levels, categorized into high and low expression based on the median expression levels, with clinicopathological factors and overall survival (OS) of these patients was assessed. RESULTS: The low-WARS1 expression group had significantly higher serosal invasion, lymph node metastasis, lymphatic invasion, venous invasion, and pathological stage than did the high-WARS1 expression group. OS was significantly worse in the low- than in the high-WARS1 expression group (5-year survival 52.2% vs. 75.9%; p=0.0001). Furthermore, in multivariate analysis, low WARS1 expression was an independent predictor for poor OS (hazard ratio=2.101; 95% confidence interval=1.328-3.322; p=0.002). CONCLUSION: In patients with locally advanced GC, after curative resection, WARS1 expression in GC tissue may be a useful prognostic marker.

Kathy Acker's sex negativity.

This essay situates Kathy Acker's work in the feminist sex wars debate of the 1980s. I suggest that the critique of Acker's work as a "nihilist version of the personal is political" is not ungrounded but might more usefully be understood as a "sex negativity" that emerges from specific feminist avant-garde literary devices. I discuss Acker's early texts, "Politics" (1972) and "Stripper Disintegration" (1973) to show how sexuality defines Acker's esthetic and political project. I consider the (negative) feminist reception of Acker's work, lay out how Acker was involved in the pornography debate, and I bring Acker's work into conversation with Andrea Dworkin's thought. The essay argues that Acker's pseudo-autobiographical strategies and montage techniques pose a problem for the feminist politicizing of self-knowledge and the genre of autobiography as a privileged site of identity formation and emancipation. In the reordering of materials, by way of replacing, exchanging, and negating, transformation is made possible by the act of rewriting's capacity to reveal substitutability. Acker's "nihilist" feminist politics challenge the self-determination and authenticity often assumed in the politicizing of lived experience. I also suggest that "the lesbian" functions as a phantasmatic figure in Acker's early work to circumvent the subject-object logic of the pornographic imagination. In short, Acker's early work illuminates the complex relation between sexuality, self-objectification, and the act of writing itself. With Acker's pseudo-autobiographical texts we can conceive of a sex negativity that is not anti-sex but challenges what Michel Foucault calls the "monarchy of sex" through non-positive affirmation.

Tryptophanylation of insulin receptor by WARS attenuates insulin signaling.

Increased circulating amino acid levels have been linked to insulin resistance and development of type 2 diabetes (T2D), but the underlying mechanism remains largely unknown. Herein, we show that tryptophan modifies insulin receptor (IR) to attenuate insulin signaling and impair glucose uptake. Mice fed with tryptophan-rich chow developed insulin resistance. Excessive tryptophan promoted tryptophanyl-tRNA synthetase (WARS) to tryptophanylate lysine 1209 of IR (W-K1209), which induced insulin resistance by inhibiting the insulin-stimulated phosphorylation of IR, AKT, and AS160. SIRT1, but not other sirtuins, detryptophanylated IRW-K1209 to increase the insulin sensitivity. Collectively, we unveiled the mechanisms of how tryptophan impaired insulin signaling, and our data suggested that WARS might be a target to attenuate insulin resistance in T2D patients.

Highly secreted tryptophanyl tRNA synthetase 1 as a potential theranostic target for hypercytokinemic severe sepsis.

Despite intensive clinical and scientific efforts, the mortality rate of sepsis remains high due to the lack of precise biomarkers for patient stratification and therapeutic guidance. Secreted human tryptophanyl-tRNA synthetase 1 (WARS1), an endogenous ligand for Toll-like receptor (TLR) 2 and TLR4 against infection, activates the genes that signify the hyperinflammatory sepsis phenotype. High plasma WARS1 levels stratified the early death of critically ill patients with sepsis, along with elevated levels of cytokines, chemokines, and lactate, as well as increased numbers of absolute neutrophils and monocytes, and higher Sequential Organ Failure Assessment (SOFA) scores. These symptoms were recapitulated in severely ill septic mice with hypercytokinemia. Further, injection of WARS1 into mildly septic mice worsened morbidity and mortality. We created an anti-human WARS1-neutralizing antibody that suppresses proinflammatory cytokine expression in marmosets with endotoxemia. Administration of this antibody into severe septic mice attenuated cytokine storm, organ failure, and early mortality. With antibiotics, the antibody almost completely prevented fatalities. These data imply that blood-circulating WARS1-guided anti-WARS1 therapy may provide a novel theranostic strategy for life-threatening systemic hyperinflammatory sepsis.

Challenges for the Pro-Life Movement in a Post-Roe Era.

This article argues that state laws banning abortions for disability violate reproductive justice for parents with disabilities. These bans deprive people with disabilities of choices that may be important to their possibilities of becoming parents, including possibilities for abortion of pregnancies that have become risky to continue. Far from protecting disability civil rights, these state law bans restrict the abilities of people with disabilities to choose to have children and to parent.

Implanting rich autobiographical false memories: Meta–analysis for forensic practice and judicial judgment making

Objective: The implanting of rich autobiographical false memories is crucial for judicial decision–making, and the forensic evaluation of a testimony. In order to assess this issue, a meta–analytical review of the probability of implanting rich autobiographical false memories was performed. Method: A total of 30 primary studies analysing the probability of implanting rich autobiographical false memories were retrieved. Random–effects meta–analyses correcting the effect size for sampling error were performed. Results: The results revealed a significant, positive, generalizable (the lower limit for the 80% credibility value was d = 1.13), and more than large mean effect size (d = 1.43[1.33, 1.53]) for the implanting of false memory. The moderating effects of stimulus type showed that the effect of the probability of implanted false memory was significantly higher in experienced events (d = 2.03[1.63, 2.43]) than in false narratives (d = 1.35[1.23, 1.47]), and in doctored photographs (d = 1.29[1.06, 1.52]). A similar effect for memory implantation was observed in both the underage (d = 1.44[1.29, 1.59]), and in adults (d = 1.36[1.22, 1.50]). The moderator techniques for implanting false memories revealed a significantly lower probability of implanting false rich memory with non–directive instructions (d = 0.90[0.53, 1.27]) than with guided imagery (d = 1.45[1.32, 1.58]), or with pressure to answer (d = 1.56[1.17, 1.95]) instructions. The event emotional valence moderator exhibited the same effect for positive (d = 1.27[1.09, 1.45]) and negative valence events (d = 1.30[1.17, 1.43]). Conclusions: The implications of the results for forensic testimony evaluation, police interrogations, and judicial cross–examination are discussed. (AU)

COGNITIVE ASPECTS OF DEPRESSION IN VICTIMS OF RADIATION ACCIDENTS, THE COVID-19 PANDEMIC, AND WARS. / КОГНІТИВНІ АСПЕКТИ ДЕПРЕСІЇ У ПОСТРАЖДАЛИХ ПРИ РАДІАЦІЙНИХ АВАРІЯХ, ПАНДЕМІЇ COVID-19, ВІЙНАХ.

The relevance of the work is due to the significant increase in depression with cognitive disorders (CD) worldwide, and in Ukraine in particular, in victims of emergencies situations (ES), and the importance, but insufficient development of clinical and psychopathological aspects of medical and psychological-psychiatric care for this contingent of people. Despite the modern achievements of psychiatry in the study of depression in ES, an important problem is CD, which worsen the quality of life of patients, increase the risk of relapse, progressive course, and suicidal behavior. In order to improve the level of psychological and psychiatric care for depressed patients with CD in post-stress disorders by radiation accidents, the COVID-19 pandemic, and wars, the study of domestic and foreign literature on these disorders, which have both common features and certain differences, was conducted.Conclusions. The study confirms the significant prevalence of depression with CD in victims of radiation accidents, the COVID-19 pandemic, and wars, the insufficient development of clinical and psychopathological aspects and theneed for further study of this problem, as well as the creation of state measures of psychological and psychiatric care for these patients.

Estimating the impact of the outbreak of wars on financial assets: Evidence from Russia-Ukraine conflict.

This study analyzes the performance of the Shanghai Composite Index, S&P 500 index, WTI oil price, and LBMA gold price when wars took place, especially the Russia-Ukraine conflict. We employ empirical methods to explore the stability, instantaneous shock, and short-term shock regarding the abovementioned financial assets. We first adopt the event study method to ascertain whether the cumulative abnormal returns of the selected assets are significant when wars break out. Then, we use the permutation test to examine the significance of price level changes. Results show that only the Shanghai Composite Index is relatively stable. Second, the difference-in-differences model indicates that the 3 unstable assets all suffered positive shocks in their price levels within several days after the Russia-Ukraine conflict broke out. The parallel trend test confirms the validity of establishing the difference-in-differences model. Third, regression discontinuity is designed to measure the impact in a longer event window, suggesting the robustness of conclusions of the difference-in-differences model and revealing an upward trend before the conflict and a downward trend after the conflict of the financial assets. The study suggests that investors consider adjustments to investment strategies and governments take precautions to diminish the risk of the outbreak of wars.

Governing digital crisis responses: platform standards and the dilemma of COVID-19 contact tracing.

In response to the impact of the SARS-CoV-2 (COVID-19) pandemic, various developers turned to smartphone-based contact tracing to address the challenges of manual tracing. Due to the presence of network effects, i.e., the effectiveness of contact tracing applications increases with the number of users, information technology standards were critical to the technology's success. The standardization efforts in Europe led to a variety of trade-offs concerning the choice of an appropriate technological architecture due to the contradictory tensions resulting from the dualism between the need for contact tracing data to contain the pandemic and the need for data minimization to preserve user privacy. Drawing predominantly on the software platform and standards literature, we conduct an interpretive case study to examine the emergence and consequences of this multi-layered decision situation. Our findings reveal how Google and Apple were able to limit the individual leeway of external developers, thereby effectively resolving the European standards war. Furthermore, we identify and discuss the various short-term and long-term trade-offs associated with the standardization of contact tracing applications and translate our findings into recommendations for policy makers with respect to future crisis situations. Specifically, we propose a strategy grounded in our data that enables responsible actors to make goal-oriented and rapid decisions under time constraints.

GLOBAL MENTAL HEALTH ENLIGTENMENT2.0 IN ACTIONFOR GLOBAL SUSTAINABILITY, PEACE AND EMPATHIC CIVILIZATION.

Mental health as a crucial global public good requires action of all scientific, political, social and cultural disciplines and sectors in our VUCA (volatile, uncertain, complex, ambiguous) world. Empathy based on love as life philosophy and a way of being in the world is the most essential and basic core element in human communications which leads to healthy, creative, flourishing, and well-functioning families, communities, nations, societies, and civilizations. Culture of empathy is an essential part of the new 2.0 Enlightenment which is a mental, social, spiritual and political movement that inspires aspiration towards global human spirit, collective mind and humanistic self, public and global mental health, cosmopolitism and empathic civilization.

The Balkan Wars in prof. Precechtel's memories.

Professor Precechtel's memories of the Balkan Wars of 1912-1913 have various facets and encompass different impressions and phases of these events. They trace the preparations in Prague and the journey to Montenegro, the ancient city of Cetinje among the poor and primitive people, experiences in the Albanian environment plagued by diseases, and the war experienced in Bulgaria. Czech doctors enthusiastically aided their Slavic brethren in the Balkans, who fought for liberation and rights. Initially, they were received coolly and with reservation as the local population had no experience with Czech doctors. However, over time, the medical expeditions proved their worth. Albania presented many obstacles, including a shortage of medicines and suitable food. The army suffered from droughts and exhaustion, leading to high mortality rates and infections. Medical activities were carried out under difficult conditions, lacking hygiene and basic supplies. Despite all the difficulties, the doctors strove to provide help, and their work was invaluable. Memories of the Balkan War are filled with stories of scarcity, challenges, human suffering, and determination to aid and solidarity.

A scoping review linking early childhood caries to violence, neglect, internally displaced, migrant and refugee status.

BACKGROUND: The aim of the scoping review was to identify and synthesize the available literature concerning the relationship between the status of refugees, migrants, and internally displaced persons (IDPs) and Early Childhood Caries (ECC) as it relates to the United Nation's Sustainable Development Goal 16 (SDG 16). METHODS: Data regarding the links between the status of refugees, migrants, and internally displaced persons (IDPs) and Early Childhood Caries (ECC), and the associations between ECC and maternal and child exposure to physical and sexual abuse, insecurity, crime, exploitation, torture, and displacement were extracted. The search was carried out in January 2023 across three databases (PubMed, Web of Science, and Scopus). Only publications in English with accessible full texts were included. Descriptive statistics were utilized to summarize the categories of the retrieved papers, and graphical representation was employed for visualization purposes. The relationships between the publications and each of the 10 targets of Sustainable Development Goal 16 (SDG 16) were also assessed. RESULTS: Forty-five studies were reviewed. Most studies (42.2%) originated from the Americas Regions, while no studies were identified from the Africa Region. A significant portion (46.7%) of the papers focused on abuse, violence, and neglect as risk factors for ECC. Migrants, refugees, and IDPs were the most investigated populations (44.4%). Only one study specifically focused on IDPs and migrants respectively. The prevalence of untreated caries was higher among migrants, refugees, and IDPs compared to the host community, ECC was more prevalent among children who experienced abuse, neglect, or were in protective care. The was no clear direction on the associations between ECC and intimate partner violence, adverse childhood experiences, and wars. In terms of the SDGs, the reviewed publications addressed four targets (SDG16.1, SDG16.2, SDG16.3, and SDG16.5) out of the ten targets outlined in SDG 16. CONCLUSION: There is available evidence regarding the connections between ECC and war, refugees, migration, violence, and neglect, as outlined in SDG 16. Future studies are needed to investigate how forced movements directly affects ECC status, how disruptions of peace and stability is a risk factor for ECC, and the associations between ECC and other indicators related to SDG 16 targets.

Impact of wars and natural disasters on emerging and re-emerging infectious diseases.

Emerging Infectious Diseases (EIDs) and Re-Emerging Infectious Diseases (REIDs) constitute significant health problems and are becoming of major importance. Up to 75% of EIDs and REIDs have zoonotic origin. Several factors such as the destruction of natural habitats leading humans and animals to live in close proximity, ecological changes due to natural disasters, population migration resulting from war or conflict, interruption or decrease in disease prevention programs, and insufficient vector control applications and sanitation are involved in disease emergence and distribution. War and natural disasters have a great impact on the emergence/re-emergence of diseases in the population. According to a World Bank estimation, two billion people are living in poverty and fragility situations. Wars destroy health systems and infrastructure, curtail existing disease control programs, and cause population movement leading to an increase in exposure to health risks and favor the emergence of infectious diseases. A total of 432 catastrophic cases associated with natural disasters were recorded globally in 2021. Natural disasters increase the risk of EID and REID outbreaks by damaging infrastructure and leading to displacement of populations. A Generic National Action Plan covering risk assessment, mechanism for action, determination of roles and responsibilities of each sector, the establishment of a coordination mechanism, etc. should be developed.

Acculturation strategies as predictors of fandom identification in the fanfiction, Star Wars fan, and furry communities.

Research suggests that people at the interface of two different cultures may face a dilemma regarding how or whether to adopt aspects of the new culture in light of their existing cultural identity. A growing body of research in fan communities suggests that similar group processes may operate in recreational, volitional identities. We tested this by examining the associations between acculturation attitudes and identification with fan communities across three studies. Fanfiction fans, Star Wars fans, and furries completed measures of four different acculturation strategies with respect to managing their fan and non-fan communities as well as a measure of their identification with the fan community. Results across the three studies consistently found that integration and assimilation strategies positively predicted fan community identification, while separation and marginalization strategies negatively predicted fan community identification. Together, the results conceptually replicate and find evidence for the acculturation model.

Distribution, preparedness and management of Ukrainian adult refugees on dialysis-an international survey by the Renal Disaster Relief Task Force of the European Renal Association.

BACKGROUND: Due to the Russian-Ukrainian war, some of the about 10 000 adults requiring dialysis in Ukraine fled their country to continue dialysis abroad. To better understand the needs of conflict-affected dialysis patients, the Renal Disaster Relief Task Force of the European Renal Association conducted a survey on distribution, preparedness and management of adults requiring dialysis who were displaced due to the war. METHODS: A cross-sectional online survey was sent via National Nephrology Societies across Europe and disseminated to their dialysis centers. Fresenius Medical Care shared a set of aggregated data. RESULTS: Data were received on 602 patients dialyzed in 24 countries. Most patients were dialyzed in Poland (45.0%), followed by Slovakia (18.1%), Czech Republic (7.8%) and Romania (6.3%). The interval between last dialysis and the first in the reporting center was 3.1 ± 1.6 days, but was ≥4 days in 28.1% of patients. Mean age was 48.1 ± 13.4 years, 43.5% were females. Medical records were carried by 63.9% of patients, 63.3% carried a list of medications, 60.4% carried the medications themselves and 44.0% carried their dialysis prescription, with 26.1% carrying all of these items and 16.1% carrying none. Upon presentation outside Ukraine, 33.9% of patients needed hospitalization. Dialysis therapy was not continued in the reporting center by 28.2% of patients until the end of the observation period. CONCLUSIONS: We received information about approximately 6% of Ukrainian dialysis patients, who had fled their country by the end of August 2022. A substantial proportion were temporarily underdialyzed, carried incomplete medical information and needed hospitalization. The results of our survey may help to inform policies and targeted interventions to respond to the special needs of this vulnerable population during wars and other disasters in the future.

A scientometric and descriptive review on the debate about repressed memories and traumatic forgetting.

Recent work suggests that the debate surrounding repressed memory and traumatic forgetting continues today. To further investigate this debate, we performed preregistered scientometric analyses on publications on the debate about repressed memory to provide information about its bibliometric evolution. Furthermore, we reviewed these publications to highlight the different positions taken by scholars on this debate. We reviewed 434 publications extracted from Scopus and Web of Science from 1969 to 2022. Our scientometric analyses permitted us to visualize the development of the publications on repressed memories and identify the terminology used to label this phenomenon. We identified three waves of publications (i.e., 1994-2000; 2003-2009; 2012-2021) showing that there is a recent peak of scholarly attention into this topic. 40% of scholars supported the phenomenon of repressed memory while 29% did not. Moreover, although in the last wave of publications, 35% of articles included critical arguments against the existence of repressed memory, a sizable number of publications (21%) supported ideas in favour of repressed memory. Finally, we observed that the term dissociative amnesia is another expression used to refer to the phenomenon. Our results provide additional evidence that the debate on repressed memories (and dissociative amnesia) is far from being over.

Is physiotherapy a luxury? What can the perplexing absence of the physical therapies tell us about the profession's future?

Physiotherapists interested in the profession's future have turned in recent years to historical evidence to understand how the physical therapies were practiced before the advent of modern healthcare. However, studies to date suggest that their practice was largely confined to social elites, and those from working-class or poor populations rarely, if ever, experienced them. To test this theory further, this study focuses on British sailors during the Napoleonic wars (1803-1815). Utilizing historical and semi-fictional accounts, this study shows that healthcare on board naval fighting ships concentrated almost entirely on the prevention of disease, and the medical and surgical management of acute trauma. Even though sailors experienced shocking levels of traumatic injury, none appear to have experienced any form of physical therapy. This study supports the argument that prior to the 20th century, the physical therapies were luxuries available primarily to those with surplus time and money, and that widespread access to physiotherapy has relied on state-sponsored universal health coverage. It follows, then, that the decline of universalized healthcare may have profound implications for many marginal groups in society, as well as the physiotherapy profession itself.